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Measure your Vitamin D levels from home to support bone strength, immune defense, and energy balance.

Vitamin D Test

Vitamin D is more than just the “sunshine vitamin.” It plays an essential role in calcium absorption, bone strength, immune regulation, inflammation control, and even emotional wellbeing. Low levels have been linked to increased risk of infections, poor mood, fatigue, and long-term bone loss. Despite its importance, Vitamin D deficiency is one of the most widespread nutritional gaps globally — affecting people in all climates and lifestyles.

Whether you spend most of your time indoors, live in a region with limited sunlight, have darker skin, follow a vegan or dairy-free diet, or simply want to optimize your health, knowing your Vitamin D status is the first step.

Our Vitamin D Test offers a fast, convenient, and scientifically validated way to measure your body’s Vitamin D stores using a small finger-prick blood sample collected at home. The test measures 25-hydroxyvitamin D [25(OH)D], the clinically recognised marker for Vitamin D status, and is analyzed using liquid chromatography–mass spectrometry (LC-MS/MS) for gold-standard accuracy.

Why Test Vitamin D?
  • Vitamin D supports bone strength, muscle function, immunity, and mental wellbeing. It regulates calcium absorption, reduces inflammation, and plays a protective role in immune defense and chronic disease prevention.
  • Deficiency is common, even in sunny regions — especially if you have darker skin, limited outdoor time, wear sunscreen daily, or follow a plant-based or dairy-free diet.
  • Low levels are linked to fatigue, poor mood, frequent illness, and brittle bones. Testing gives clarity on whether your intake (from sun, food, or supplements) is sufficient.
  • Vitamin D Plus goes further by measuring your Vitamin D Metabolite Ratio (VMR), which tells you how well your body is metabolising and activating Vitamin D. This deeper insight helps identify inefficiencies or overactive conversion that basic Vitamin D tests can’t detect.
  • Testing empowers smarter decisions about your health — helping you adjust your supplement regimen, track your progress, and personalize your approach for long-term wellness.
How to Improve Your Vitamin D
  • Sunlight: 10–30 minutes several times per week
  • Food sources: fatty fish (salmon, sardines), fortified milks, cereals, eggs
  • Supplementation: D3 (cholecalciferol) is most effective; daily needs often range from 600–1000 IU
What Is a Healthy Vitamin D Range?

A healthy Vitamin D level is considered to be between 30–50 ng/mL, with the broader clinical reference range spanning 20–80 ng/mL. Levels within the optimal range are associated with improved calcium absorption, stronger bones, better immune function, and reduced risk of chronic illness.

When Vitamin D levels drop below 30 ng/mL, the body may struggle to maintain bone density, modulate immune responses, or regulate inflammation. In contrast, excessively high levels (typically above 80–100 ng/mL) may lead to toxicity, particularly when driven by over-supplementation.

The Vitamin D Plus Test also evaluates your Vitamin D Metabolite Ratio (VMR) — a key marker of how efficiently your body is processing Vitamin D. A VMR between 4.3%–14.4% indicates optimal metabolism. Values outside this range may suggest poor activation or excessive breakdown of Vitamin D, guiding more personalized interventions.

Backed by Science
  • Holick et al. (2011): Established 30–50 ng/mL as the optimal serum range to support bone health and immune function.
  • Pludowski et al. (2013): Recommended population-wide Vitamin D screening, especially for those at risk of deficiency.
  • Shieh et al. (2017): Identified the clinical value of metabolite ratio testing (VMR) in assessing individual Vitamin D metabolism.
  • Lips et al. (2019): Linked suboptimal Vitamin D levels to increased risk of fractures, infections, and inflammatory disease.

These studies support the role of Vitamin D testing in both identifying deficiency and optimising function — especially when combined with metabolic insights provided by advanced tests like Vitamin D Plus.

Key Benefits
  • Strengthen Bone and Muscle Health: Vitamin D supports calcium absorption and is essential for maintaining bone density and muscle function.
  • Boost Immune Defense: Plays a critical role in regulating immune responses and reducing inflammation.
  • Personalise Supplementation: See if your current routine (sun exposure, diet, or supplements) is effectively supporting your levels.
  • Measure Metabolism with Vitamin D Plus: Go beyond basic testing — assess how well your body converts and utilises Vitamin D with the Vitamin D Metabolite Ratio (VMR).
  • Track Progress Over Time: Recheck levels every 3–6 months to confirm your efforts are working — especially when making dietary or supplement changes.
  • Easy At-Home Collection: No clinic visit required — a simple finger-prick blood sample is all it takes.
  • Peace of Mind: Make confident, informed decisions about your immune, bone, and metabolic health with clinically validated data.
What's Measured?
  • 25-hydroxyvitamin D [25(OH)D]: This is the main circulating form of Vitamin D and the most accurate indicator of your overall Vitamin D status.
  • Vitamin D Metabolite Ratio (VMR) (Vitamin D Plus only): This advanced biomarker measures how efficiently your body is converting Vitamin D into its active forms. It provides insight into your metabolic processing, not just your total levels. VMR is calculated as the ratio of 24,25(OH)₂D₃ to 25(OH)D₃
Who Should Use This Test?
  • Individuals with limited sun exposure or darker skin
  • People experiencing fatigue, low mood, or frequent illness
  • Those following vegan, dairy-free, or low-fat diets
  • Adults supplementing with Vitamin D and seeking confirmation of effectiveness
  • Healthcare professionals monitoring bone health, immune resilience, or Vitamin D metabolism
What's Included?
  • Dried blood spot (DBS) collection card
  • Lancets, alcohol swab, gauze, bandage
  • Prepaid return envelope and instructions
  • Accredited lab analysis + secure online results
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FAQs

Why test Vitamin D?

Vitamin D is essential for strong bones, immune health, and overall wellbeing — yet deficiency is common and often goes unnoticed. Testing your Vitamin D levels helps identify whether your current intake from sunlight, diet, or supplements is sufficient.

With Vitamin D Plus, you also gain insight into how well your body is metabolising Vitamin D through the Vitamin D Metabolite Ratio (VMR)— providing a fuller picture of both status and function.

Do I need to fast?

No, fasting is not required. You can test at any time of day, but avoid high-dose Vitamin D supplements for 12–24 hours before testing.

What is an optimal Vitamin D Levels?

25(OH)D (Vitamin D): Optimal range is 30–50 ng/mL (reference range: 20–80 ng/mL)

VMR (Vitamin D Plus only): Optimal range is 4.3–14.4%, indicating healthy Vitamin D metabolism

Low levels may lead to fatigue, low mood, weakened bones, and poor immune resilience. High levels may increase the risk of toxicity, especially with supplementation.

Is this test suitable for vegetarians or vegans?

Yes. This test is well suited for individuals on plant-based or vegan diets, who may be at higher risk of Vitamin D deficiency due to limited dietary sources. Testing helps determine whether you are getting sufficient Vitamin D from fortified foods, sun exposure, or supplements — and whether additional support is needed.

When is the best time to test?

You can take this test at any time of day. No fasting is needed. For the most accurate results, we recommend avoiding Vitamin D supplements for 12–24 hours before sample collection.

How can I increase my Vitamin D?

You can raise your Vitamin D levels through a combination of sun exposure, diet, and supplementation:

  • Sunlight: Aim for 10–30 minutes of midday sun, several times per week (adjust for skin tone, geography, and season)
  • Food sources: Fatty fish (salmon, mackerel, sardines), egg yolks, and fortified foods like cereals, milk, and orange juice
  • Supplements: Vitamin D3 (cholecalciferol) is typically more effective than D2; common doses range from 600–1,000 IU/day depending on needs

Monitoring your levels with follow-up testing can help ensure your efforts are working.

Is the test safe and easy to do at home?

Yes. The test uses a simple dried blood spot (DBS) collection method — just a few drops from your fingertip, no clinic visit or blood draw required. All supplies and step-by-step instructions are included in the kit.

What does the test include

Your kit includes everything you need to collect and return your sample from home: a dried blood spot (DBS) collection card, full instructions, and a return envelope. You’ll also receive a secure lab report with your Vitamin D results and reference ranges.

How accurate is the Vitamin D test from a dried blood spot?

Our Vitamin D and Vitamin D Plus tests are analyzed using liquid chromatography–mass spectrometry (LC-MS/MS) — the gold-standard method for measuring 25(OH)D and its metabolites. This approach delivers high sensitivity and clinical precision from just a few drops of blood.

To ensure consistent and reliable results, our lab participates in the DEQAS proficiency testing program (Vitamin D External Quality Assessment Scheme), an internationally recognised benchmark for Vitamin D testing accuracy.

How long until I get results?

Once your sample is received by the lab, your results will be available within 3-5 business days.