NAD+ (nicotinamide adenine dinucleotide) is a critical molecule that powers metabolism, supports DNA repair, and regulates aging pathways. Often referred to as the “metabolic spark plug,” NAD+ levels decline with age, stress, poor sleep, and illness—contributing to fatigue, cognitive decline, and impaired cellular function.

Our NAD+ Index Test provides a non-invasive way to measure your body’s NAD+ status using just a few drops of blood from a dried blood spot (DBS) sample. It’s ideal for anyone interested in longevity, cellular health, or tracking the effects of NAD+ precursors like NR (nicotinamide riboside) or NMN (nicotinamide mononucleotide). This test provides a comprehensive profile of the nicotinamide adenine dinucleotide (NAD) system, including both oxidised and reduced forms of NAD+ and NADP. It helps assess your body's cellular energy potential, redox balance, and vitamin B3 metabolism.

We measure the following from a dried blood spot (DBS) sample:

• NAD⁺ (oxidised form) – nmol/mL
• NADH (reduced form) – nmol/mL
• NADPH – nmol/mL
• NADP⁺ – nmol/mL
• Nicotinamide (NAM) – nmol/mL
• Nicotinic Acid (NA) – nmol/mL
• NAD/NADH Ratio
• NADP/NADPH Ratio
• NAD/NADP Index – also known as the “niacin number”

These markers give deep insight into mitochondrial health, oxidative stress balance, and whether your NAD+ precursors (like NMN or NR) are effectively supporting your metabolism.

• Supports healthy aging – NAD⁺ is essential for DNA repair, telomere maintenance, and activating longevity genes like sirtuins.
• Boosts cellular energy – Crucial for ATP production inside mitochondria.
• Informs supplement use – Helps assess whether you’re responding to NAD⁺ precursors like NMN, NR, or niacinamide.
• Detects nutrient imbalances – Understand how your body is utilizing vitamin B3 and whether there’s a need to adjust intake.
• Optimizes redox balance – The NAD⁺/NADH and NADP⁺/NADPH ratios give insight into your oxidative stress and detoxification capacity.
• Empowers smarter health planning – Track changes over time and personalise your supplementation, fasting, or metabolic strategy.

You can naturally support or enhance your NAD⁺ levels with a combination of lifestyle interventions, dietary strategies, and targeted supplementation:

• Exercise regularly
  Aerobic exercise and resistance training both stimulate NAD⁺ biosynthesis and support mitochondrial health.
• Try intermittent fasting or caloric restriction
  Fasting activates AMPK and SIRT1 pathways, which help conserve and regenerate NAD⁺ inside your cells.
• Eat NAD⁺-supportive foods
  Include foods rich in tryptophan and vitamin B3: turkey, chicken, mushrooms, peanuts, whole grains, salmon, and green vegetables.
• Limit alcohol and ultra-processed foods
  These deplete NAD⁺ stores by increasing oxidative stress and overburdening detox pathways.
• Supplement wisely
  Common NAD⁺ precursors include: Nicotinic Acid (NA)
• Sleep well & reduce chronic stress
  NAD⁺ is consumed during cellular repair. Prioritizing quality sleep and stress recovery helps maintain levels over time.
• Monitor and retest
  Re-checking your NAD⁺ Index after 6–12 weeks can help assess the impact of supplements or lifestyle changes.

• NAD⁺: 30–80 µmol/L
• NADH: 5–15 µmol/L
• NAD⁺/NADH ratio: 2.5–8.0
• NADP⁺/NADPH ratio: 0.5–1.5
• NAD/NADP Index: 0.8–1.2 (optimal range)

Low NAD⁺ levels or a low NAD⁺/NADH ratio may indicate oxidative stress, mitochondrial dysfunction, or niacin insufficiency. A skewed NAD/NADP Index can signal problems with vitamin B3 metabolism or poor response to supplementation.

• Yoshino et al. (2018): Demonstrated that declining NAD⁺ levels are a hallmark of aging and metabolic dysfunction, impacting mitochondrial health and insulin sensitivity.
• Rajman et al. (2018): Reviewed how NAD⁺ metabolism influences SIRT1, DNA repair, inflammation, and cellular resilience — making it a central node in aging and disease prevention.
• Liu et al. (2021): Highlighted the role of NAD⁺/NADH and NADP⁺/NADPH ratios in cellular redox balance and energy metabolism, with implications for neurodegeneration, fatigue, and immune health.
• Trammell et al. (2016): Developed quantitative methods to measure NAD⁺ and its metabolites in blood, establishing reference ranges for NAD⁺ status across age groups.

These studies validate the clinical relevance of monitoring NAD⁺ metabolites and redox ratios as emerging biomarkers of metabolic health, cellular energy, and aging. The NAD⁺ Index Test offers a powerful way to assess your biochemical resilience and tailor your longevity strategy with precision.

• Measure cellular energy at its source – Evaluate NAD⁺ and related coenzymes powering your mitochondria
• Track supplementation impact – Know whether NMN, NR, or niacin are actually improving your NAD status
• Optimize metabolic resilience – Understand your redox balance, detoxification potential, and mitochondrial function
• Support longevity planning – Use real data to guide anti-aging strategies backed by science
• Easy at-home collection – No blood draw needed

Your report includes absolute concentrations (µmol/L) and ratios:

• NAD⁺ – Nicotinamide adenine dinucleotide (oxidised form)
• NADH – Reduced form, involved in electron transport and redox cycling
• NADP⁺ / NADPH – Supports anabolic reactions and antioxidant defenses
• NAM (Nicotinamide) – A primary form of vitamin B3
• NA (Nicotinic Acid) – Another form of vitamin B3
• NAD⁺/NADH Ratio – Indicator of mitochondrial redox balance
• NADP⁺/NADPH Ratio – Reflects antioxidant regeneration potential
• NAD/NADP Index (Niacin Number) – Composite biomarker of total niacin pool and cellular redox status

• Individuals supplementing with NMN, NR, or niacin-based compounds
• People focused on anti-aging, longevity, and mitochondrial health
• Athletes and biohackers interested in energy output and recovery
• Individuals with chronic fatigue, poor sleep, or brain fog
• Anyone curious about how diet, fasting, or stress affects NAD+ dynamics

• Dried blood spot (DBS) collection card
• Lancets, alcohol swab, gauze, bandage
• Prepaid return envelope and instructions
• Accredited lab analysis + secure online results